Lentiviral vectors
Fastrack your lentiviral vector generation with our optimized DNA bricks.
Overview
Third generation lentiviral vectors have become extremely popular to stably integrate transgenes in eukaryotic cells.
At Polyplus, we have optimized lentiviral pTransgene for third generation lentivirus systems, to be fully compatible and readily available for vector assembly.
Such bricks have been biologically validated in mono- or bi-cistronic set-up and routinely used to generate tailor-made transfer plasmids/vectors.
Do remind that for optimal packaging of virions, the size of the cargo, from LTR to LTR should amount to 8-9kb. If needed, longer cargo can be assembled into our vectors
Please note that we do not provide the service of viral particle production and the plasmid packaging for lentiviral vectors, but you can contact us if you want to optimize yours!
However, you can use our vectors to produce viral particle or transfer it to any suitable service provider. As part of our collaboration, we can always recommend you to skilled professional, the Viral Vectors platform of the GIGA Institute in Liège.
Key advantages of Polyplus Lentiviral pTransgene:
- Optimized lentiviral DNA bricks containing all the genetic features required in a transfer plasmid for third generation lentivirus systems.
- The optimal cargo size recommended (from LTR to LTR) is 8-9kb : up to 4 ORFs under control of two independent promoters driving mono or bi-cistronic transcripts (2A peptides, IRES).
- Biologically validated in mono- or bi-cistronic set-up and routinely used to generate tailor-made transfer vector.
- e-Zyvec service by Polyplus allows to generate different size of cargo simultaneously and to compare them to select the highest titer configuration.
- eZ-LV pTransgen are fully compatible with regular production protocols.
- From validation by customers of final sequence to delivery of prototypes: 2 to 4 or 4 to 6 weeks depending on option chosen (premium vs standard).
PricinG
Our service prices will be based on the specifications of your project. One of the first specification will be the complexity of your DNA vectors (Regular, advanced, sophisticated), then the type of services (standard or premium)…
As your understand, tailored services also means tailored fees. If you wish to have a precise price of your project in euros, you will must to create your project on our online platform and submit it to our experts (see below how to do it), or you can also contact us.
How to order
LOG INTO OUR ONLINE PLATFORM
CREATE YOUR OWN PROJECT
SUBMIT YOUR PROJECT
FAQ & DOCUMENTATION
Faq
Our lentiviral vectors are designed and optimized to be used in third lentiviral generation system.
For an optimal packaging of virions, the size of the cargo, from LTR to LTR should amount to 8-9kb. If needed, longer cargo can be assembled into our vectors.
The eZ-Lentivirus-5-arm includes the 5′ LTR (truncated) sequence, as well as the HIV-Psi, the RRE; the cPPT/CTS, the T promoter and the RSV promoter sequences.
The ez Lentivirus-3-arm includes the PPT, the delta U3 and the HIV-1 5’LTR regulatory elements followed by a SV40-PA sequence.
e-Zyvec provides the full plasmid sequence once our design is validated by yourself. After the plasmid production, we validate the sequence using NGS technology, and we provide you the report of produced plasmids.
After validation of your vector designs with our experts, primers and synthesis will be ordered to produce your vectors. Upon material reception we guarentee 2-4 weeks for plasmid delivery with our premium service, and 4-6 weeks with the regular way.
If you have problems creating your project or drawing your vector, you can consult our tutorial here, or book a meeting with our experts.
The way we work is based on regular exchange with the customers. There is a lot of back and forth to ensure that we have the right pieces of information from you, and that what we want to offer actually fits your needs. If we put it in a nutshell, it can be summarized as the big plasmid schema in our Home page.
It’s not strictly a milestone scheme, but everything is not done in one go. All along the project, you get to ask for modifications if needed. We are working in close relationship with you to conduct the project.
There are general terms and conditions that are provided with the quote. Amongst them, there is a restricted use of the plasmid constructs for Research purpose.
The quote is split between the achievements (there are several lines, additional or not) and it is a one-time payment. Generally, payment is due during the beginning of the project (after discussing your needs and what we can offer you).
e-Zyvec – 80 rue du Docteur Yersin
59120 Loos
FRANCE
+33 (0)3 59 61 50 43
