Today, we would like to highlight the study of Dr Drider (University of Lille) on new Class II bacteriocins, that was published last June. We are happy to have efficiently contributed to this project aiming at the discovery of new antibiotic alternatives, by providing a set of 10 DNA constructs assembled in less than 4 weeks.
Having identified 5 ORFs of interest using bioinformatic, he asked us to generate expression vectors for these ORFs, tagged either at their Nterminal or Cterminal position.
As shown in the figure, each purified ORF was found to be active against the targeted bacterial strain. Furthermore, N-tagged peptides were more efficient than C-tagged ones, demonstrating an unpredictable side effect of the position of the Tag, but also confirming that the peptides worked independently of the tagging.
This could only be concluded by testing both tagging strategies simultaneously. And this is exactly what our services and technology can bring to this kind of project.