Not everybody needs to sequence full genomes...
In order to improve the quality control of our modular DNA vectors, we developped our in-house sequencing pipeline.
Based on Illumina technology and the use of an I-seq100 apparatus, we are currently finalizing our QC process so we can sequence fully multiple DNA vectors in one shot.
The expertise we developed in this field can be applied to multiple type of ‘low-volume’ projects of sequencing, for which larger sequencing platform might not be optimal in terms of costs and delays.
Here is a non-exhaustive list of such projects:
- Vector Sequencing: we can compare your sequencing results to theoretical maps and highlight any differences, or de novo assemble NGS reads to provide you a map of an unknown (forgotten) plasmid.
- Large Molecule Sequencing: sub-genomic molecules like Bacterial Artificial Chromosomes (BAC) or Yeast Artificial Chromosome (YAC) can be sequenced using a multiple index strategy to ensure coverage.
- Variant Calling: can be applied to libraries of vectors or amplicons (from genomic DNA for example) to assess and quantify the genetic variablity within a population of molecules, clones or cells.
For any of these projects we can work with small series of samples (1 to 96).