Not everybody needs to sequence full genomes...
In order to improve the quality control of our modular DNA vectors, we developped our in-house sequencing services pipeline.
Based on Illumina technology and the use of an I-seq100 apparatus, we are currently finalizing our QC process so we can sequence fully multiple DNA vectors in one shot with 100% coverage.
We provide our illumina iseq-100 to ensure your high throughput sequencing:
• 1.2gb max output
• 4 millions reads per run
• 2 x 150 bp max read length
The expertise we developed in this field can be applied to multiple type of ‘low-volume’ projects of sequencing, for which larger sequencing platform might not be optimal in terms of costs and delays.
Here is a non-exhaustive list of such projects:
- • Plasmid Sequencing: we can compare your sequencing results to theoretical maps and highlight any differences, or de novo assemble NGS reads to provide you a map of an unknown (forgotten) plasmid.
- • Large Molecule Sequencing: sub-genomic molecules like Bacterial Artificial Chromosomes (BAC) or Yeast Artificial Chromosome (YAC) can be sequenced using a multiple index strategy to ensure coverage.
- • Variant Calling: can be applied to libraries of vectors or amplicons (from genomic DNA for example) to assess and quantify the genetic variablity within a population of molecules, clones or cells.
For any of these projects we can work with small series of samples (1 to 96).