We can assemble any relevant DNA sequences together, as long as the nucleotide content is known. In this way, we can contemplate creating virtually any well-thought-out DNA vector, so that it will convey the right genetic program to the right living system with the right means of control (e.g. transcription, integration, regulation).
As your scientific needs are not standard, you may have looked in vain at the standard vector lists of your regular providers. However, we can now create those personalized vectors you really need.
On our dedicated technology page, you will find some examples of how our service might help you.
Genome editing projects
Genome editing projects
We can help you to assemble your CRISPR/Cas9 derived sequences in optimised vectors
CRISPR/Cas9-based systems are revolutionising molecular biology, with new applications blooming every year since 2013 (first use reported in mammalian cells).
Basic CRISPR-based tools are distributed by regular reagent providers. However, you might prefer to use personalised systems to fulfil your scientific goals.
We can help you to assemble your CRISPR/Cas9-derived sequences in optimised vectors:
To screen multiple sgRNA effect using a single DNA molecule
To express Cas9, stably or transiently, together with selected reporter proteins (fluorescent or luminescent proteins)
To fuse Cas9 or Cas9-derived proteins with other protein domains
To adapt the CRISPR/Cas9 system in your cellular model (codon optimisation, cell specific promoters, etc.).
Protein engineering projects
One of the key advantages of our DNA assembly technology is that we completely control, down to a single nucleotide, the way that DNA bricks are clicked to each other. This means that our assembly can not only be seamless, but also that we can introduce point mutations into a sequence anywhere we want.
This makes e-Zyvec’s technology the perfect tool for protein engineering by simultaneously managing:
Point mutations, single and/or multiple
Epitope tagging, at either or both ends of your proteins, but also anywhere inside your sequence of interest with respect to the translation’s frame
Fusion and deletion of protein domains, to create protein chimeras relevant to your research.
Also, it is worth keeping in mind that all these aspects can be managed while designing the regulatory elements of your vectors (promoters, terminators, selection markers etc.). This means that any composite coding sequence you have created with us can be easily re-assembled afterwards in a variety of expression vectors to fit all the cellular systems you need it to.
As functional genetics and biotechnology evolve, there is a constantly increasing need to simultaneously induce and assess the effect of multiple genes of interest. Of course, one can envisage introducing each genetic modification in a living system one-by-one to obtain the desired cellular model. However, limitations due to available selection markers can rapidly impede such a strategy, which, in itself, remains tedious and resource-consuming.
There are real advantages, therefore, both technical and scientific, in introducing several transgenes at once into a cellular model.
IRES and 2A-like peptide sequences are two existing tools that allow dual expression of proteins from a single transcript. Of course, e-Zyvec’s vectors can also be assembled to contain such sequences.
However, e-Zyvec’s technology can be used to create vectors containing multiple complete expression cassettes (promotors + ORF + terminators), each cassette being completely customisable within the vector. This allows each transgene expression to be controlled independently by picking the appropriate promoter (artificial or natural).
There is a tremendous range of possible applications of our technology. Some examples of vectors we have designed include:
Engineering of metabolic pathways,
Visualization of subcellular compartments,
Combination of inductive (cDNA) and repressive (shRNA) cassettes.
Co-design your very specific tools with us
Of course, you may have a very precise idea of what you need in your vectors, or at least of what you want your vectors to do.
However the vector that will fulfil your requirements is nowhere to found in the regular place, and you are not quite sure how to build it by yourself…
We will work with you to design your very own molecular tools and make sure that we can get the parts to bring together to produce your molecules