Today, we would like to highlight the study of Dr Denis Mottet (Gene Expression and Cancer Laboratory, GIGA, Université de Liège) on the Identification of a new role for the TFIP11 protein in the regulation of spliceosome activity.
We are happy to have contributed to this project aimed at investigating the importance of TFIP11 in pre-RNA splicing and its consequences in maintaining genome stability.
In order to study the interaction of TFIP11 with the Coilin, plasmids encoding different constructs of TFIP11 cDNA in fusion with FLAG were generated by e-Zyvec
As shown on the figure, the N-ter region of TFIP11, but also the patch G domain is required for interaction with the Coilin. This has been demonstrated with the truncation/deletion of different sequences of the TFIP11 cDNA in our constructs and it is a good example of what our services and our technology can bring to this type of project.
Thanks to Dr Mottet for this wonder ful project and his trust!